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KMID : 0950619950040010057
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Journanl of Allied Health Sciences
1995 Volume.4 No. 1 p.57 ~ p.65
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An Assessment of Performance Characterestics for Na, K and Cl Analysis by Enzymatic Assays
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Lee Chang-Kyu
Lee Seung-Gwan
Ryu Jung-Rok
Kim Jai-Young
Lee Kook-Sung
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Abstract
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In spite of the clinical importance and the frequency with which serum electrolytes are measured in clinical laboratories, few assay methods have been appropriate for automated chemistry analyzers.
Currently available electrolyte assays for routine laboratories fall into four categories : flame photometry, ion-selective electrodes, colorimetry and coulometric titration.
Recently, new enzymatic methods for, Na, K and CI(Boehringer Mnnheim, Germany) have been proposed.
We evaluated the analytical performance, and performance characterestics of the Hitachi 747 serum Na, K and Cl enzymatic assays as compared to the ASTRA assays.
For Na, comparison of the enzymatic method with the ion-selective electrode on 30 actual patient specimens showed a correlation of 0.986, a slope of 0.789 with an intercept of 23.28.
For K, comparison of the enzymatic method with the ion-selective electrode on 30 actual patient specilens showed a correlation of 0.999, a slope of 0.908 with an intercept of 0.549
For Cl, comparison of the enzymatic method with the coulometry on 30 actual patient specials showed a correlation of 0.985, a slope of 1.356 with an intercept of -29.590.
Based on the data presented in this study, new enzymatic assays for some electrolytes have demonstrated its ability to provide clinically useful data over a broad ranges.
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